Design of Peptide Ligand for Lactoferrin and Study of Its Binding Specificity

The in silico modelling of peptides complementary to lactoferrin was carried out using the Protein 3D software package and replication natural bonding site between pneumococcal surface protein (PSP) (LF). modeling based on analysis conjugated ion–hydrogen bond systems these proteins (CIHBS). oligopeptide EEVAPQAQAKIAELENQVHRLE proposed via computer synthesized solid phase synthesis technique, purified, analyzed with MS HPLC methods confirm >95% purity. peptide then studied by capillary electrophoresis (CE). CE experiments demonstrated split zone presence LF, due complex formation subsequent mobility change system peptide-protein. reference homomyeloperoxidase myoglobin did not show binding LETI-11.